Composite
NG7

Part:BBa_K2623027:Design

Designed by: Yunyun Hu   Group: iGEM18_XMU-China   (2018-10-02)


siRNA with C/Dbox RNA structure to bind with L7Ae (BBa_K2623026).


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1268
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1298
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1304
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

The coded RNA may be too short and form secondary structure.


Source

Partly from E.coli's genomic sequence.


References